The game will hit retail shelves on April 21, 2011 for PS3, 360, Mac and PC.
"Portal 2 is Valve's most innovative title in the company's history and will be launched with a first-class launch campaign this April," said Doug Lombardi, vice president of marketing at Valve.
"We're excited to be working with Electronic Arts to deliver this title as we expand our platform strategy to include the Mac and PlayStation 3 platforms."
Portal 2 was named the most anticipated game of 2011 at the Spike TV Video Game Awards earlier this month.
It will see players introduced to a host of fresh puzzles and new test chambers in never-before-seen areas of Aperture Science Labs. Players will also be reunited with GLaDOS, the occasionally murderous computer companion from the original Portal.
Meanwhile, co-op mode will feature its own unique campaign and characters, and will force players to both act and think together.